Once you have your experimental results (e.g., absorbance from an ELISA or radioactivity from a binding assay), you can use the GraphPad Prism desktop software to turn those values back into molarity units. Step 1: Interpolating from a Standard Curve
In pharmacology and biochemistry, molarity is often used to find the of a drug:
If you don’t log-transform, Prism will fit a hyperbola (not sigmoid) and fail. molarity graphpad
: Quickly check the concentration of a solution you have already prepared. Dilute a Stock Solution : Solve the
Click Analyze and select Interpolate a Standard Curve . For many biological assays, a Sigmoidal, 4PL (four-parameter logistic) model is the industry standard. Once you have your experimental results (e
Master Your Solution Prep: Using the GraphPad Molarity Calculator
This increases power and reduces ambiguity. Dilute a Stock Solution : Solve the Click
In this example, the graph would show a linear relationship between concentration and absorbance, with the equation of the line shown above.