Beyond basic band measuring, Gel-Pro Analyzer offers a suite of advanced tools designed for specific scientific needs:
Identifying the size of a molecule is fundamental. Gel-Pro allows users to define a "Standard Lane" containing a molecular weight ladder. The software then interpolates the position of unknown bands against this standard to calculate their molecular weight with high precision. Furthermore, by comparing the optical density (intensity) of an unknown band against a standard of known concentration, the software can calculate the absolute mass of the sample.
Analyzing PCR products and RFLP (Restriction Fragment Length Polymorphism) patterns for genetic mapping or forensics.
The quality of your digital analysis depends heavily on the initial gel preparation. gel-pro analyzer
Gel-Pro Analyzer is versatile enough to support a wide range of molecular biology workflows:
Estimating the size and concentration of DNA/RNA fragments in agarose gels. Standard Workflow for Gel Analysis
: Use a gel documentation system, like the GelPro System , to capture high-resolution images (up to 5MP) under UV or white light. 2. Software Workflow (Gel-Pro Analyzer) Beyond basic band measuring, Gel-Pro Analyzer offers a
: Always include a molecular weight marker (ladder) to calibrate your results later in the software.
Anyone who has run a distorted SDS-PAGE or a warped agarose gel knows the frustration: standard rectangular bounding boxes simply don't work. Gel-Pro’s and automatic lane straightening are legendary. The software doesn't just draw a box; it follows the actual path of the sample, correcting for smiling or wavy bands without manual pixel-by-pixel editing.
Users can calculate molecular weights by comparing unknown bands against established standards. Furthermore, by comparing the optical density (intensity) of
Import an image from a scanner, digital camera, or gel documentation system.
: Load consistent volumes in each well to allow for accurate quantitative comparison.
When you are unsure if a faint band is a true positive or just smeared background, the (grey value topography) is invaluable. A quick glance at the z-axis spike tells you if the signal-to-noise ratio passes muster. This feature alone saves researchers from running unnecessary western blot replicates.
The output is highly customizable. Researchers can generate reports that include the gel image, the annotated analysis (with bands labeled and measured), and the raw data tables. These can be exported directly into spreadsheets or scientific publication software.