Mt-lbv |link| Instant
Recombinant MTLV nucleoprotein shows no serological cross-reactivity with Heartland virus or SFTSV in ELISA, allowing specific serodiagnosis.
Maximum-likelihood tree (based on L segment) places MTLV in the , but as a basal sister lineage to a subclade containing Heartland virus isolates from Missouri and Oklahoma. Estimated divergence time: ~300–500 years ago, long predating human population expansion in the region. mt-lbv