Qiagen Buffer Recipes

SDS solution. . If SDS precipitates, warm to 37°C before use . Buffer N3 (Neutralization Buffer)

Below are the common Qiagen Buffer Recipes used for plasmid purification and DNA cleanup: Recipe (per 1 Liter) P1 Resuspension 50 mM Tris-HCl (pH 8.0), 10 mM EDTA, 100 μg/mL RNase A . P2 200 mM NaOH, 1% SDS. P3 Neutralization 3.0 M Potassium acetate (pH 5.5). QBT Equilibration qiagen buffer recipes

SDS solution. . If SDS precipitates, warm to 37°C before use . Buffer N3 (Neutralization Buffer)

Below are the common Qiagen Buffer Recipes used for plasmid purification and DNA cleanup: Recipe (per 1 Liter) P1 Resuspension 50 mM Tris-HCl (pH 8.0), 10 mM EDTA, 100 μg/mL RNase A . P2 200 mM NaOH, 1% SDS. P3 Neutralization 3.0 M Potassium acetate (pH 5.5). QBT Equilibration